河南科技大学学报（自然科学版）2012，Vol.33Issue(3)：50-53,57,5.
南阳黄牛CD14基因扩增及序列分析
Amplification and Sequence Analysis of CD14 Gene in Nanyang Yellow Cattle
摘要
Abstract
The CD14 gene in Nanyang yellow cattle was segmently amplificated by polymerase chain reaction (PCR) method. Primers were designesd according to CD14 gene sequence of Holstein in GenBank. The complete sequence of 2 969 bp, containing complete coding region of CD14,5 ' and 3 ' non-coding region, were obtained. Sequence analysis showed that CD14 gene in Nanyang Yellow cattle contains ORF of 966 bp in length encoded 321 amino acids,comprised of A( 18.4% ) ,T( 18.4% ) ,C(32. 9% ) ,G(30. 2% ). 2 bases of coding region were changed compared to CD14 gene of Holstein, but none of amino acid changed. 7 bases of non-coding region at the end of 5 ' and 3' were changed compared to CD14 gene of Holstein. The homology rates of nucleotide sequences of CD14 gene among Nanyang yellow cattle and Holstein cow, buffalo, sheep, goats,pigs, monkeys, gorillas, human, mouse were decreased in tum, and were 99. 8% , 98. 2% , 96. 8% , 92. 8% ,83. 5% ,19. 8% , 79. 7% , 79. 5% , 71. 9% respectively. The results of categorize from phylogenetic tree were similar to the traditional classification results. There is no transmembrane domains by means of protein structure prediction of CD14 gene sequence in Nanyang yellow cattle.关键词
南阳黄牛/CD14/序列分析Key words
Nanyang yellow cattle /CD14/ Sequencing分类
农业科技引用本文复制引用
马蓓蓓,龙塔,薛云,周峰,赵战勤..南阳黄牛CD14基因扩增及序列分析[J].河南科技大学学报（自然科学版）,2012,33(3):50-53,57,5.基金项目
国家自然科学基金项目(31001051) （31001051）
