林业科学2012，Vol.48Issue(3)：31-37,7.
茶树2个MYB转录因子基因的克隆及表达分析
Cloning and Expression of Two MYB Transcription Factors in Tea Plant (Camellia sinensis)
摘要
Abstract
MYB transcription factors represent a family of genes that include the conserved MYB DNA-binding domain, and they are widely involved in the regulation of plant development and secondary metabolism. In this study, Part of sequences of two MYB transcription factors was determined through the cDNA microarray hybridization and selection of cDNA library derived from tender shoots. The full-length cDNAs of the genes were obtained with RT-PCR and RACE, and they were 1 132 bp and 1 020 bp, named as CsMYBl and CsMYB2 ( GenBank accession No. HQ660373 and HQ660374) , and contained ORFs of 879 bp and 675 bp encoding 292 and 224 ami no acids, respectively. Sequences analysis showed that the deduced protein molecular weight of the two genes were 32.9 ku and 25.4 ku, and the proteins contained two conserved MYB domains near the N-tenninus and a conserved Cl motif near the R3 domains. The deduced amino acid sequence of CsMYBl and CsMYB2 from tea plant showed high identity with that of other plants, for instance CsMYBl shared 57% homology with MYB1 of Gossypium hirsutum and CsMYB2 shared 75% homology wilh MYBC2 of Vitis vinifera. The result of real time-PCR analysis showed the two genes were expressed constitutively in all tissues with different expression levels, e. g. the relative expression level of CsMYB2 in leaf was hundred times higher than that in root. Additionally, shading enhanced CsMYBl expression, while the treatment did not alter the expression level of CsMYB2.关键词
茶树/MYB转录因子/基因克隆/表达分析Key words
tea plant (Camellia sinensis)/ MYB transcription factors/ gene cloning/ expression分类
农业科技引用本文复制引用
马春雷,姚明哲,王新超,金基强,陈亮..茶树2个MYB转录因子基因的克隆及表达分析[J].林业科学,2012,48(3):31-37,7.基金项目
"国家茶叶产业技术体系"项目(CARS-23) （CARS-23）
国家自然科学基金项目(31100504 （）
31170624 （）
30901159) （）
浙江省自然科学基金项目(Y3100291 （）
Y3090041:Y3110260). （）
