林业科学2012，Vol.48Issue(3)：105-109,5.
球孢白僵菌侵染光肩星天牛幼虫的扫描电镜及组织病理观察
Pathological Observation of Anoplophora glabripennis Larva Infected by Beauveria bassiana by Using SEM and Light Microscope
摘要
Abstract
The infection course of Beauveria bassiana in the integument of Anoplophora glabripennis larva was examined by the scanning electronic microscope ( SEM ) , and a histopathological study with paraffin section of (he midgut of the host was also conducted by using a light microscope. The result revealed that the germ tube with its length more than 3. 08μm was able to penetrate the integument in 48 h post inoculation. Then the germ tube gradually developed into hypha and new conidium. Some silk-like and net structures were found in the late phase, which had not been reported before. Most of the conidia invaded the cuticle of the host larvae directly with the germ tube, and sometimes an appressorium was produced during the course of infection and invaded the cuticle later. Microscope examination with paraffin section showed that the fat body in the body cavity of the infected larva became unclear and the nucleus was atrophic in 3 days post inoculation. Vacuoles appeared in the Malpighian tubules which caosed the tubules deformed. The peritrophic membrane in the midgut was decomposed and disappeared. The circular muscle layer in the midgut folded inward and was disintegrated. The midgut cavity of the A. glabripennis became turbid.关键词
球孢白僵菌/光肩星天牛/侵染过程/病理变化/扫描电镜Key words
Beauveria bassiana/ Anoplophora glabripennis/ infection process/ histopathology/ SEM分类
农业科技引用本文复制引用
邓彩萍,闫喜中,刘红霞,郝赤,骆有庆..球孢白僵菌侵染光肩星天牛幼虫的扫描电镜及组织病理观察[J].林业科学,2012,48(3):105-109,5.基金项目
国家重点基础研究发展计划资助项目(2009CB119200) （2009CB119200）
林业公益性行业科研专项(200904029) （200904029）
中央高校基本科研业务费专项(HJ2010-12) （HJ2010-12）
山西农业大学引进人才科研启动基金(XB2009011). （XB2009011）
