林业科学2012，Vol.48Issue(6)：95-99,5.
转Bt基因南林895杨对杨扇舟蛾体内酶的影响
Effects of Bt Nanlin 895 Poplar on the Metabolic Enzymes of Scarce Chocolate Tip, Clostera anachoreta
摘要
Abstract
The larvae of scarce chocolate tip, Clostera anachoreta, were fed with leaves of Bt Nanlin 895 poplar to study effects of Bt protein on the metabolic enzymes of the pest. The results showed that the activities of eBterase and carboxylestera.se were inhibited by Bt protein. The esterase activity of the larvae reduced after being fed with the transgenic poplar leaves in 4 - 48 h and the activity of carboxylesterase rose during the initial 4 h but declined after then. The activity of multi-function oxidase in the larvae slightly declined after being fed with the transgenic leaves in 24 h, but the difference was not significant from CK. The activity of glutathione-S-transferase did not significantly change during the initial 36 h, but was inhibited by the Bt protein in 48 h. The activity of acetylcholinesterase was not affected by the feeding. The activity of superoxide dismutase rose in 12 and then declined during 12 -48 h. The activity of peroxidase rose during 4 - 48 h while the activity of catalase declined during 4 - 12 h and rose from 24-48 h. Therefore, the transgenic poplar inhibited the activities of esterase and carboxylesterase, disturbed the protect enzyme system and had an insecticidal effect of the larvae.关键词
转Bt基因南林895杨/解毒酶/保护酶/乙酰胆碱酯酶Key words
Bt Nanlin 895 poplar/ detoxification enzyme/ protect enzyme/ acetylcholinesterase分类
农业科技引用本文复制引用
张雁,郭同斌,诸葛强..转Bt基因南林895杨对杨扇舟蛾体内酶的影响[J].林业科学,2012,48(6):95-99,5.基金项目
国家“863”高技术研究发展计划项目“杨树分子育种与品种创制”(2011AA100201) （2011AA100201）
江苏省高新技术资助项目“高产、多抗转基因杨树新品系培育及开发”(BG2007314). （BG2007314）
