南京医科大学学报（自然科学版）2012，Vol.32Issue(5)：585-591,7.
甲状腺癌中类干细胞的侧群细胞的分离及鉴定
Isolation and characterization of a stem-like side population in thyroid cancer cell lines
摘要
Abstract
Objective: To identify the presence of cancer stem-like cells (side population,SP) in thyroid cancer and compare the growth pattern,clonogenity and invasive potential between cancer SP and non-SP cells. Methods; Three different human anaplastic thyroid cancer cell lines(including HTh74,SW1736,and C643) were stained with Hoechst 33342 and sorted for a small fraction of SP by fluorescence-activated cell sorting (FACS). The gene expression of stem cell marker-0ct4 (oetamer-binding transcription factor 4) and genes related to cancer resistance and relapse-ABCG2(ATP-binding cassette superfamily G member 2) and MDR1 (mullidrug resistance 1) in SP and non-SP cells was compared by performing RT-PCH and immunofluorescenl staining. Colony formation assay was performed to evaluate the self-renewal potential of SP cells,and cell invasive potential was measured by using the Matrigel invasion assay. Results: Cancer SP cells possessed some intrinsic stem cell properties as they preferentially expressed stem cell marker Oct4 and ABC transporter genes-ABCG2 and MDRl.and can generate both SP and non-SP cells in vitro. Colony formation analysis and Matrigel invasion assay revealed that these cancer SP cells were more clonogenic and displayed higher invasive potential than non-SP cells. Conclusion; These findings suggest that the SP in thyroid cancer cell lines is enriched with alem-like cancer cells thai may be the source of tumor drug resistance and recurrence.关键词
肿瘤干细胞/甲状腺/侧群细胞/ABC转运体Key words
cancer stem cells/thyroid/side population/ ABC transporter分类
生物科学引用本文复制引用
张菀姣,兰玲,郑旭琴,武晓泓,徐宽枫,蒋琳,唐伟,崔岱..甲状腺癌中类干细胞的侧群细胞的分离及鉴定[J].南京医科大学学报（自然科学版）,2012,32(5):585-591,7.基金项目
国家自然科学基金(30800416),江苏省自然科学基金(BK2008469) （30800416）
