南京医科大学学报（自然科学版）2012，Vol.32Issue(5)：592-597,6.
EP2受体介导的前列腺素E2影响胆管细胞癌细胞HuCCT1细胞间黏附因子-1表达和侵袭的研究
Prostaglandin E2 upregulates ICAM-1 expression and activity in HuCCT1 cells via the EP2 receptor signaling pathway
摘要
Abstract
Objective:To investigate the effect of prostaglandin E2 (PGE2) on the expression of intercellular adhesion molecule-1 (ICAM-1) and the cell migration ability by EP2 receptor in cholangiocarcinoma HuCCTI cells. Methods: HuCCTI cells were treated with PGE2,EPl-4 receptor agonist,EP2 receptor antagonist, AC inhibitor,and protein kinase A (PKA) inhibitor. Western blotting and scratch assay were employed to detect the protein level of ICAM-1 and the cell migration ability in HuCCTI cells. Results; PGE2 might upregulate the protein level of ICAM-I in cholangiocarcinoma HuCCTI cells. The protein level of ICAM-1 was increased by 66.17%(P < 0.05) compared with the control group after treatment with 5 μmol/L PGE3 for 24 h,and the increase was dose- and time-dependent. The cell migration ability of HuCCTI was increased by 43.29% (P < 0.01) compared with the group after treated with 5 μmol/L PGE2. The protein expression of ICAM-1 in HuCCTI cells were increased by 257.88%(p< 0.05) after treatment with EP2 receptor agonist for 24 h.and the cell migration ability of HuCCTI was increased by 56.99%(p < 0.01). The protein level of I-CAM-1 decreased by 49.14% (P < 0.05) compared with the group,which were treated with PGE2 after treatment with 10 (μmol/LEP2 receptor antagonist,and the cell migration ability of HuCCTl decreased by 52.06% (P < 0.01). When treated with 25 μmnl/L AC inhibitor SQ22536 and 10 μmol/L PKA antagonist H89 for 24h,lhe protein levels of ICAM-I were decreased by 72.87%(P < 0.05),80.78%(p< 0.05) compared with the group treated with EP2 receptor agonist. Conclusion; PGK2 upregulates the protein level of ICAM-I and the cell migration ability through EP2 receptor in cbolangimarcinoma HuCCTl cells,which could be partly related to the cAMP-PKA signaling pathway.关键词
胆管细胞癌/前列腺素E2/EP2受体/cAMP-PKA/细胞间黏附因子-1Key words
cholangiocarcinoma/PGK2/EP2 receptoncAMP-pKA/ICAM-l分类
生物科学引用本文复制引用
许燕,白小明,张丽,马娟,张海,汪亦品,冷静..EP2受体介导的前列腺素E2影响胆管细胞癌细胞HuCCT1细胞间黏附因子-1表达和侵袭的研究[J].南京医科大学学报（自然科学版）,2012,32(5):592-597,6.基金项目
国家自然科学基金(30871015,81172003) （30871015,81172003）
