南京医科大学学报（自然科学版）2012，Vol.32Issue(5)：604-608,5.
骨髓间充质干细胞对HepG2肝癌细胞增殖的影响
The effects of bone marrow-derived mesenchymal stem cells on proliferation of human hepatocellular carcinoma cell line HepG2 in vitro
摘要
Abstract
Objective; To investigate the effects of rat bone marrow-derived mesenchymal stem cells (MSCs) on the proliferation and cell cycle distribution of human hepatocellular carcinoma cell line HepG2 in vitro. Methods: Rat bone marrow-derived mesenchymal stem cells were isolated and identified by FACS and were expanded ex vino and the supernatant wag collected. Cell growth was measured by MTT assay,colony formation and soft agar colony formation assay. Cell cycle progression was analyzed by flow cytometry. Western blot assays were used to detect the expression of Cyclin Dl. Results: Mesenchymal stem cells promoted the proliferation and clonogenicity of cell line HepG2 indirectly by MSC-CM(P < 0.05). Cell number in G0/G1 phase was decreased and that in S and G2/M phase was increased (P < 0.05). MSCs promote the expression of protein Cyclin Dl in HepG2 (P < 0.05). Conclusion: Mesenchymal stem cells can promote the proliferation of hepatocellular carcinoma cell line by upregulating Cyclin Dl.关键词
间充质干细胞/肝癌细胞/增殖/Cyclin D1Key words
mesenchymal stem cells/ hepaiocellular carcinoma cell/proliferation/Cyclin Dl分类
生物科学引用本文复制引用
唐甜,李红,薛冰,唐小军,陈汐敏,仇镇宁,朱进,Tom C Tsang,冯振卿..骨髓间充质干细胞对HepG2肝癌细胞增殖的影响[J].南京医科大学学报（自然科学版）,2012,32(5):604-608,5.基金项目
美国Cure Cancer Worldwide corporation资助项目,江苏省科技支撑计划资助项目(BE2011842) （BE2011842）
