南京医科大学学报（自然科学版）2012，Vol.32Issue(5)：641-644,4.
重组高迁移率族蛋白B1对肺上皮细胞细胞因子释放的影响及其机制
Effects of recombinant HMGB1 on the release of proinflammatory cytokines in lung epithelial cells and its mechanism
摘要
Abstract
Objective:Tn investigate the effects of high mobility group box I (high mobility group boxl.HMGBl) on the extracellular release of proinflammatory cytokines in lung epithelial cells,and its possible mechanism. Methods;The cytokines levels of tumor necrosis factor-α (TNF-α),interleukin(IL)-lβ,IL-6 in the culture supematants of BEAS-2B human lung epithelial cells was determined by RLISA assay after induction with different concentrations of recombinanl HMGM protein (1,10,100 and 1000 μg/L). The inhibitory effect of Toll-like receptor 4 (TLR4) antibody (10 mg/L) on the release of these cytokines was ohserved. Results: Recombinant HMGB1 dose-dependently increased the levels of TNF-α,IL-1β and IL in the culture supematants of BEAS-2B cells. The levels of IL-1β and IL-6 in the culture supernatanls were lime-dependently increased,and TNF-α level reached to the peak after 6 h induction of 100μg/L HMGB1. TLR4 antihody partly inhibited HMGB1-induced release of TNF-α,Iβ and IL-6 (P < 0.05 or P < 0.01). Conclusion: HMGB1 can induce lung epithelial cells to release TNF-α,IL-1β and ll.-6,and TLR4 may be involved in the regulatory mechanism.关键词
高迁移率族蛋白B1/上皮细胞/Toll样受体4/细胞因子Key words
HMGB1 protein/epithelial cells/Toll-like receptor 4/cytokines分类
生物科学引用本文复制引用
吴国荣,王发龙,陈文,陈静瑜,刘洁,王琼,赵琪,陈国千..重组高迁移率族蛋白B1对肺上皮细胞细胞因子释放的影响及其机制[J].南京医科大学学报（自然科学版）,2012,32(5):641-644,4.基金项目
国家自然科学基金(81070074) （81070074）
南京医科大学科技发展基金重点项目 (2010NJMUZ59) （2010NJMUZ59）
