畜牧与饲料科学2012，Vol.33Issue(5)：3-6,4.
一株抑菌芽胞杆菌的分离鉴定及产细菌素性能检测
Isolation and Identification of a Bacillus Strain with Bacteriostatic Ability and Detection on Its Characters of Producing Bacteriocin
辛国芹 1徐海燕 1武香玉 1汪孟娟 1谢全喜 1谷巍1
作者信息
- 1. 山东宝来利来生物工程股份有限公司,山东泰安271000
- 折叠
摘要
Abstract
A strain BX-6 with good bacteriostatic activity was isolated from fresh chicken manure by routine microbiological method. Based on the means of colony morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence phylogeny analysis, the strain was identified and classified. And the antimicrobial characters of bacteriocin produced by BX-6 was detected by cylinder-plate method. The results showed that strain BX-6 was identified as Bacillus subtilis. Some antibacterial substance of H202 was produced in the fermentation process of BX-6. The bacteriocin had a good thermal stability,pH tolerance and it was sensitive to most proteases. And it had better antibacterial effects on Salmonella pullorum C79-13 ,chicken Escherichia coil O1 and Staphylococcus aureus C56011. So it had the potential to be used as feed additive of livestock and poultry.关键词
枯草芽胞杆菌/分离鉴定/系统发育分析/细菌素/抑菌特性Key words
Bacillus subtilis/isolation and identification/phylogenetic analysis/bacteriocin/antimicrobial characters分类
生物科学引用本文复制引用
辛国芹,徐海燕,武香玉,汪孟娟,谢全喜,谷巍..一株抑菌芽胞杆菌的分离鉴定及产细菌素性能检测[J].畜牧与饲料科学,2012,33(5):3-6,4.
