农业工程学报2012，Vol.28Issue(10)：287-292,6.DOI:10.3969/j.issn.1002-6819.2012.10.045
白酒糟水解液摇瓶发酵生产木糖醇的工艺优化
Process optimization for producing xylitol by shaking-flask fermentation from distiller's grains hydrolysates
摘要
Abstract
Xylitol was produced from hydrolysates of distiller's grains by Candida tropicalis 1779, and the optimum conditions of the process were found by single-factor experiment and orthogonal test. The fermentations were conducted in 250 mL flasks. The results showed that the satisfied fermentation effect was attained under the technical conditions of inoculum age 27 h, inoculating volume 20mL, liquid volume in flask 100 mL, the addition of nitrogen source 20 mL and nitrogen concentration 48 g/L. Under these conditions, the concentration of xylitol and residual xylose were 11.85mg/mL and 463.51 μg/mL in the fermentation broth, respectively. Under the optimum conditions, the utilization rate of xylose and reducing sugar are 45.62% and 74.81%, respectively. Fermentation experiments showed that it was feasible to produce xylitol by fermentation using lees hydrolysates as a carbon source.关键词
酒/发酵/工艺控制/水解液/热带假丝酵母/木糖醇Key words
wine/ fermentation/ process control/ hydrolysates/ Candida tropicalis/ xylitol分类
轻工纺织引用本文复制引用
任海伟,张飞,张轶,陈晓前,石进才,王永刚,李志忠..白酒糟水解液摇瓶发酵生产木糖醇的工艺优化[J].农业工程学报,2012,28(10):287-292,6.基金项目
甘肃省青年科技基金计划(1107RJYA065) （1107RJYA065）
甘肃省教育厅研究生导师基金(0803-08) （0803-08）
教育部大学生创新性实验计划项目资助(101073131) （101073131）
