农业生物技术学报2012，Vol.20Issue(5)：529-535,7.DOI:10.3969/j.issn.1674-7968.2012.05.009
黑番鸭血管活性肠肽受体-1基因(VIPR-1)的cDNA克隆及其组织表达特性分析
cDNA Cloning of Vasoactice Intestinal Peptide Receptor-1 Gene (VIPR-1) and Its Expression Characteristics in Tissues of Black Muscovy Duck ( Cairina moschata)
摘要
Abstract
Recent studies have already indicated that vasoactice intestinal peptide (VIP) is closely related to the habit of birds' nesting. In this study, we first cloned VIP receptor gene and analyzed its characteristics of expression in different tissues of muscovy duck. Reversed transcript PCR was used to clone vasoactice intestinal peptide receptor-1 (VIPR-1) gene from the hypothalamus of black muscovy ducks(Cairirna moschata), the cDNA sequence of this gene consisted of 1 125 nucleotides, which coded 335 amino acids (GenBank accession No. JN625215). The sequence of VIPR-1 was highly conserved in avian species. The muscovy duck VJPR-] shared 96% amino acid identity to that of chicken (Gallus gallus), turkey (Meleagris gallopavo), and quail (Coturnix japonica), while it shared only 70%~72% amino acid identing to that of some mammalians like human(Homo sapiens), mice(Mus musculus), pig(Sus scrofa) and cattle(Bos taurus). The significant differences were detected in levels of VlPR-1 expressed in the stage of egg-laying, incubating and out-of-lay (PO005) by Real-time PCR, and the highest expression level was in the stage of incubating, while the lowest was in out-of-lay. This finding indicated that expression level VIPR-] was closely correlated with the reproduction stages. In addition, VIPR-J mRNA was found to be expressed in the hypothalamus, pituitary and ovary of black muscovy ducks. The highest expression level of VlPR-1 was found in hypothalamus, while the lowest level was in ovaries (P<0.01). In summary, VIPR-1 gene is highly conservative, and its tissue-specific expression patterns imply that VIPR-l gene is involved in the role of hypothalamus-pituitary-ovary axis in regulating the incubation of muscovy ducks.关键词
黑番鸭/血管活性肠肽受体基因(VIPR-1)/就巢/实时荧光定量PCRKey words
Black muscovy duck/ Vasoactice intestinal peptide receptor-1 gene (VIPR-1)/ Incubating/ Real-time quantitative PCR引用本文复制引用
郑嫩珠,陈晓燕,陈晖,朱志明,缪中纬,辛清武,王正朝,卢立志..黑番鸭血管活性肠肽受体-1基因(VIPR-1)的cDNA克隆及其组织表达特性分析[J].农业生物技术学报,2012,20(5):529-535,7.基金项目
福建省科技计划项目(No.2010R1025-6) （No.2010R1025-6）
国家现代农业产业技术体系建设专项(No.nycytx-45-06) （No.nycytx-45-06）
