中国海洋大学学报（自然科学版）2012，Vol.42Issue(5)：68-73,6.
草皮氏海参皂苷单体抗血管新生的实验研究
The Experimental Study of Pearsonothuria graeffei saponins Inhibiting Angiogenesis Induced by Tumor Cells
摘要
Abstract
To investigate the effects of echinoside A(EA)and dsechinoside A(DSEA)which were extracted from Pearsonothria graeffei on anti-angiogenesis, we MTT method and AO/EB staining were med to observe the influence of EA and DSEA on HUVEC cell proliferation and apoptosis. In addition, in vitro angiogenesis assay was applied to determine the effect of angiogenic ability of endothelial cells, also chick embryo chorioallantoic membrane (CAM) model was used to check whether the neovascularization of CAM could be suppressed in vivo. The result indicated that EA and DSEA could decrease proliferation and induce apoptosis of HUVEC in dosage and time dependent manners. Moreover, both of them could inhibit the neovascularization in vitro and suppress angiogennesis of CAM in vivo. It is suggested that EA and DSEA can obviously inhibit the neovascularization and the inhibitory effect partially due to their structure.关键词
草皮氏海参皂苷/细胞凋亡/血管新生/鸡胚尿囊膜/血管内皮细胞Key words
Pearsonothuria graeffei saponins/ cell apoptosis/ neovascularization/ CAM/ HUVEC分类
生物科学引用本文复制引用
杨玉红,王静凤,赵芹,张珣,李兆杰..草皮氏海参皂苷单体抗血管新生的实验研究[J].中国海洋大学学报（自然科学版）,2012,42(5):68-73,6.基金项目
海洋公益性行业科研专项(201105029) （201105029）
国家自然科学基金项目(30972284) （30972284）
国际科技合作项目(2010DFA31330) （2010DFA31330）
"泰山学者"建设工程专项 （）
浙江省重大科技专项(2008C02009)资助 （2008C02009）
