热带作物学报2012，Vol.33Issue(1)：20-23,4.DOI:10.3969/j.issn.1000-2561.2012.01.005
木薯叶片染色体制片技术研究
Technique for Microscopical Slides of Cassava Leaf for Chromosome Observation
摘要
Abstract
The young leaves of Manihot esculenta Crantz were used to study the effects of sampling time, pretreatment chemical solution, fixed solutions, hydrolysing time and staining on the chromosome observation slides. The results showed that the optimal sampling time of leaves was about 8:30~10:00 a.m. The samples pretreated with a solution of 0.1% colchicine and 0.002 mol/L 8-hydroxyquinoline at room temperature for 3 hours and fixed with the fixative solution(alcohol : chloroform : glacial acetic acid = 6:3:1), and then hydrolysed with 1 mol/L HCI for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution were proved to be nice for the microscopical slides preparation of Cassava Leaf for Chromosome Observation.关键词
木薯/嫩叶/染色体/制片技术Key words
Manihot esculenta Crantz/ Leaf/ Chromosome/ Microscopical slides分类
轻工纺织引用本文复制引用
张健,郭军辉,陈雄庭,王颖,王文泉,赖杭桂,吴坤鑫,倪彦艳,黄琴..木薯叶片染色体制片技术研究[J].热带作物学报,2012,33(1):20-23,4.基金项目
国家"973"计划"木薯淀粉高效累积途径及其关键基因"项目(No.2010CB126601). （No.2010CB126601）
