热带作物学报2012，Vol.33Issue(1)：137-142,6.DOI:10.3969/j.issn.1000-2561.2012.01.028
螺旋粉虱体内共生菌的研究
Studies on the Symbiotic Bacteria of Spiralling Whitefly
摘要
Abstract
Symbionts in insects play an important role to their hosts. The symbiotic bacteria dramatically increases reproduction and survival in a common insect pest We investigated the bacteria community, Wolbachia and the effects of host plant on bacterial composition in invasive population of spiralling whitefly, which may lay foundation on the control of the insects, we compared the 16S rRNA sequences by denaturing gradient gel electrophoresis(DGGE) and 16S rRNA sequence analysis. Also Specific primers were designed to detect the Wolbachia in the invasive spiralling whitefly. Analyses of 16S rRNA genes indicated that one kind of bacterial symbiont was common in the invasive spiralling whitefly feeding on different host plants. They are primary symbionts -Candidatus Portiera aleyrodidarum belonging to the Gammaproteobacteria. There is a certain difference among the bacterial community in the spiralling whitefly feeding on different host plants. Staphylococcus sp. was present in low number in some spiralling whitefly. And no symbionts of Wolbachia were found for the invasive spiraling whilefly in Hainan Island. Special bacteria Symbionts might associate with the invasive spiraling whitefly. And bacterial symbionts varied according to the diet The Wolbachia are not harbored in this newly invasive spiraling whitefly.关键词
共生菌/16S rDNA文库/DGGE/Wolbachia菌Key words
Symbiont/ 16S rDNA libraries/ DGCE/ Wolbachia分类
生物科学引用本文复制引用
黄武仁,牛黎明,朱文静,韩冬银,张方平,符悦冠..螺旋粉虱体内共生菌的研究[J].热带作物学报,2012,33(1):137-142,6.基金项目
公益性行业(农业)科研专业经费(No.201103026) （农业）
院基金项目"海南杀虫微生物资源收集、效果评价及应用研究" （）
国家国际科技合作计划项目(No.2011DFB30040). （No.2011DFB30040）
