热带作物学报2012，Vol.33Issue(2)：296-301,6.DOI:10.3969/j.issn.1000-2561.2012.02.017
利用oligo芯片技术鉴定橡胶树死皮相关基因
Identification of Genes Associated with Tapping Panel Dryness in Hevea brasiliensis Using Oligonucleotide Microarrays
摘要
Abstract
Tapping panel dryness (TPD) is one of the main limiting factors for increasing the yield of natural rubber, and causes serious economic loss to the rubber production. In this research, the genes associated with TPD were identified by oligonucleotide microarrays hybridized with the latex from TPD and healthy rubber trees, and validated with RT-PCR method. The results from microarray analyses showed that 26 genes were differentially expressed between the TPD and healthy rubber trees. The 26 differentially expressed genes were classified into the following functional categories: rubber biosynthesis, programmed cell death, stress/defense response, metabolism of reactive oxygen species, DMA methylation, ubiquitin-proteasome pathway, cellular communication and signaling, and protein synthesis, process and transport, etc. In addition, the expression patterns of 12 differentially expressed genes randomly selected were further validated by RT-PCR analyses. This research laid foundations for identifying genes related to TPD on large scale and revealing the molecular mechanism of TPD in rubber tree.关键词
橡胶树/死皮/oligo芯片/TPD相关基因/RT-PCRKey words
Hevea brasiliensis/ Tapping Panel Dryness/ Oligonucleotide microarrays/ TPD-related genes/ RT-PCR分类
农业科技引用本文复制引用
覃碧,刘向红,邓治,李德军..利用oligo芯片技术鉴定橡胶树死皮相关基因[J].热带作物学报,2012,33(2):296-301,6.基金项目
国家自然科学基金"利用分子生物学方法研究橡胶树死皮发生分子机制"(No.30960310) （No.30960310）
海南省自然科学基金"橡胶树死皮相关基因芯片开发与应用"(No.310072) （No.310072）
中国热带农业科学院橡胶研究所基本科研业务费专项"橡胶树死皮发生关键基因HbTCTP功能研究"(No.1630022011024). （No.1630022011024）
