四川大学学报（自然科学版）2012，Vol.49Issue(3)：687-692,6.DOI:10.3969/j.issn.0490-6756.2012.03.039
大熊猫肠道抗生素耐药菌质粒的研究
Study on the plasmids of antibiotic-resistance bacteria in intestine of giant pandas
摘要
Abstract
The relationships of antibiotic-resistance bacteria in intestine of giant pandas and plasmids separated from Sichuan Wolong National Nature Reserve were analyzed. The results showed that 6 E. coli contained different sizes plasmid DNA. SDS and elevated growth temperature (43 ℃) were used to cure the plasmids. Of the 6 kinds of plasmid profiles, two plasmids were showed the disappearance of some plasmid bands. Regarding to the antibiotic-resistance level, it was found that the treatment of curing a-gents usually resulted in the decreased resistance level; even 2 lost some antibiotics resistance completely. These 6 kinds of plasmids were transformed into E. coli JM109 separately. One of them generated ampicillin-resistant transformants. The results showed that resistance of antibiotic-resistance bacteria in intestine of giant pandas were related to plasmids.关键词
大熊猫/抗生素抗性/大肠杆菌/质粒消除/转化Key words
giant panda, antibiotic-resistance, E.coli, plasmid curing, transformant分类
生物科学引用本文复制引用
严悦,余丛,张义正,汤纯香,李德生,张志和,侯蓉,谭雪梅..大熊猫肠道抗生素耐药菌质粒的研究[J].四川大学学报（自然科学版）,2012,49(3):687-692,6.基金项目
成都大熊猫繁育研究基金项目(CPF08008) （CPF08008）
