四川师范大学学报（自然科学版）2012，Vol.35Issue(4)：562-566,5.DOI:10.3969/j.issn.1001-8395.2012.04.026
杉木根际固氮菌筛选及其溶磷性与分泌IAA特性研究
Screening of Nitrogen-fixing Bacteria in Rhizosphere of Cunninghaimia Lanceolata and Investigation on Their Properties of Phosphate-solubilizing and IAA-producing
摘要
Abstract
Chinese fir ( Cunninghaimia Lanceolata) is a main tree species of planted fast-growing forest in China. The problem on rapid reduction of soil fertility resulted from nutrient consumption of Chinese fir plantation still remains to be solved since the studies on screening and application of rhizosphere bacteria of Chinese fir with growth-promoting effect are rarely reported. In our study, 16 strains of nitrogen-fixing bacteria are isolated from rhizosphere soil of Chinese fir by measurement of nitrogenase activities using acetylene reduction assay after culturing in Ashby medium. Their abilities of dissolving inorganic and organic phosphorus are evaluated by dissolving phosphate zone on Menkina media and molybdenum blue spectrophotometry. IAA-producing abilities of the strains are measurated by the Salkowski colorimetry. The results indicate that 5 strains show high potential of nitrogen fixing with nitrogenase activity of over 150 nmol · mL-1 · h-1. NGJ-4 has the highest nitrogenase activity (264.7 nmol · mL-1 · h-1) and NCX-5 takes the second place (237.4 nmol · mL-1 · h-1'). There are 8 strains with abilitiy of dissolving inorganic phosphorus. The available phosphorus increments of NGJ-8, NGJ-4 and NGX-3 are 182.7, 158.4 and 133.9 mg · L-1, respectively, which can't dissolve organic phosphorus. There are 5 strains with low dissolving organic phosphorus abilitiy. The highest available phosphorus increments derived from organic phosphorus is only 46.1 mg · L-1 of NGX-5. Most of the strains can secrete IAA. The first 5 strains have a high IAA concentration of more than 20 mg · L-1 in supernatant of bacteria solution. The IAA concentration levels of NGX-5, NGJ-3, NGJ-4 and NCX-3 are 35.8, 32.1, 27.8 and 27. 7 mg · L-1, respectively. Consequently, NGJ-4, NGX-5 and NGJ-8 integrated prominent nitrogen-fixing, phosphorus-dissolving and IAA-producing can be utilized in developing multiple-effective microbial fertilizer.关键词
杉木林/根际微生物/固氮酶活性/溶磷力/植物生长素IAAKey words
Cunninghaimia Lanceolata/ rhizosphere bacterium/ nitrogenase activity/ phosphorus solubilizing capability/ phytohormone IAA分类
农业科技引用本文复制引用
周德明,李蓉..杉木根际固氮菌筛选及其溶磷性与分泌IAA特性研究[J].四川师范大学学报（自然科学版）,2012,35(4):562-566,5.基金项目
国家林业局948项目基金(2009-4-30)和林业公益性行业科研专项基金(200904006、201004014)资助项目 （2009-4-30）
