水产学报2012，Vol.36Issue(6)：849-858,10.DOI:10.3724/SP.J.1231.2012.27768
草鱼Ⅰ型胶原蛋白α1基因cDNA全序列克隆、组织分布及其生物信息学分析
Cloning, mRNA expression and bioinformatic analysis of full length type I collagen alpha 1 cDNA from grass carp (Ctenopharyngodon idellus)
摘要
Abstract
Type I collagen, as a number of collagen family, is the most abundant collagen and major components of the extracellular matrices of all metazoan life, and plays crucial roles in differentiation, formation of collagen fibers and tissue remodeling after injury, etc. Type I collagen alpha 1(COL1A1) cDNA of grass carp (Ctenopharyngodon idella) was isolated through the RT-PCR and RACE approaches. The cDNA was 5 772-bp in length, including a 4 347-bp CDS (coding sequence) and encoded a polypeptide of 1 449 aa. The homology of COL1Al amino acid with relative species (zebrafish, goldfish, etc.) was as high as 93% with zebra fish and goldfish. The protein peptide molecular weight was 137.2 ku and theoretical pI was 5.44 using ProtParam software on line. The protein peptides of COL1A1 possessed 6 a-helixes, 12 β-sheets, others of ruleless coil regions, and 18 regions of triple helical repeats, 22 low complexity regions, 17 function domains. There were two calcium-binding sites and one zinc-binding site in the COL1A1 protein peptide. COL1A1 mRNA was determined in all the tested 8 tissues (muscle, intestine, hepatopancreas, gill, skin, fin, kidney and spleen) of grass carp by semi- quantitative RT-PCR, and the mRNAs expression in gill, kidney, skin and fin significantly higher than other tissues (P<0.05). The structure and bioinformat-ics characteristics of the COL1A1 from grass carp may help to further understand the function of COL1A1 gene in the repair process of damaged tissue in the grass carp.关键词
草鱼/Ⅰ型胶原蛋白/COL1A1/组织分布/生物信息学Key words
Ctenopharyngodon idella/ type I collagen/ COL 1A1/ mRNA expression/ bioinformatics分类
生物科学引用本文复制引用
刘邦辉,郁二蒙,王广军,余德光,谢骏,王海英,龚望宝..草鱼Ⅰ型胶原蛋白α1基因cDNA全序列克隆、组织分布及其生物信息学分析[J].水产学报,2012,36(6):849-858,10.基金项目
广东省自然科学基金自由申请项目(10151038001000004) （10151038001000004）
现代农业产业体系建设专项项目(nycytx-49) （nycytx-49）
广东省海洋渔业科技推广项目(A200901D04) （A200901D04）
