水产学报2012，Vol.36Issue(6)：937-943,7.DOI:10.3724/SP.J.1231.2012.27674
虾夷扇贝脓胞病病原的分离、鉴定与致病性
Isolation, identification and pathogenicity of Vibrio chagasii from Patinopecten yessoensis
摘要
Abstract
In recent years cultured sea scallop (Patinopecten yessoensis) dead on a large-scale in seas near Changhai County, Dalian, China, in summer. The main symptom is the abscess on adductor muscle. The transmission experience was improved to indicate that a bacterium isolated from natural infected focus, named XJ02 displayed intense pathological activity to scallops, and the symptom is the same to natural intention. The bacterium XJ02 identification test through morphological specificity, physical and chemical properties and 16S rRNA gene sequence analysis showed the bacterium is Vibrio chagasii. There has been no any research on the pathogenicity of V. chagasii. This article for the first time to find a more compre-hensive's vibrio physicochemical properties of research. The dose-and temperature-dependent test indicated that the bacterium had the most pathogenicity at 19 ℃ while the concentration was 1.09x105 CFU/mL. The medicine sensitivity analysis that's to check Ampicillin, Benzylpenicillin, Ciproflxacin etc is sensitive during 20 drugs sensitive test, to Tetracycline, Doxycycline is not sensitive element.关键词
虾夷扇贝/查氏弧菌/脓胞病Key words
Patinopecten yessoensis/ Vibrio chagasii/ abscess分类
农业科技引用本文复制引用
滕炜鸣,李文姬,张明,于佐安,李石磊,刘项峰,李华琳,付成东..虾夷扇贝脓胞病病原的分离、鉴定与致病性[J].水产学报,2012,36(6):937-943,7.基金项目
国家海洋公益性行业科研专项(200805037) （200805037）
辽宁省农业科技特派项目(2008203004) （2008203004）
