水产学报2012，Vol.36Issue(6)：958-968,11.DOI:10.3724/SP.J.1231.2012.27883
海豚链球菌感染对不同品系罗非鱼血液生化指标和肝脏HSP70 mRNA表达的影响
Studies on blood biochemical indices and expression of hepatic HSP70 mRNA of different tilapia strains artificially challenged with Streptococcus iniae
摘要
Abstract
In order to establish the superiority, four different tilapia strains (GIFT tilapia, New GIFT tilapia, Egypt Nile tilapia and Red tilapia) were compared in the study. At day 100 tilapias in the treatment group were intraperitoneally injected with Streptococcus iniae suspension (2.95×108/mL) to evaluate disease resistance of four different tilapia strains against S. iniae in terms of the serum biochemical parameters and hepatic HSP70 mRNA expression before and after infection. Another set of twenty fish taken from each tank was challenged with S. iniae using the same method as above, and the cumulative mortality of 4 strains was recorded at different times post infection. The results showed that GIFT tilapia and New GIFT tilapia were more sensitive to S. iniae at 96 h after infection, cumulative mortalities were 36.67% and 38.33% respectively. Egypt Nile tilapia was less sensitive to pathogen and no death occurred during the experiment. The levels of serum cortisol (COR) and glucose (GLU) and expression levels of HSP70 mRNA in GIFT tilapia, New GIFT tilapia and Red tilapia strains were highly significant(P<0.05), and the activities of glutamic-pyruvic transaminase (ALT), glutamic-oxaloacetic transaminase (AST)and lysozyme (LSZ) also increased, while serum alkaline phosphatase (AKP), triglyceride and cholesterol levels were significantly lower than those prior to infection (P<0.05). By utilizing the energy from resolved hepatin and lipid, the synthesis of hepatic HSP70 mRNA and levels of LSZ and globulin in Egypt Nile tilapia were improved, and thus the non-specific immunity strengthened. It is suggested that in the course of tilapia breeding, effective combination of the disease resistance with growth be conducted so as to guarantee sustainable development of the tilapia industry.关键词
罗非鱼/海豚链球菌/血液生化指标/HSP70 mRNA表达Key words
tilapia/ Streptococcus iniae/ blood biochemical indices/ HSP70 mRNA expression分类
农业科技引用本文复制引用
强俊,杨弘,王辉,徐跑,柒壮林,何杰..海豚链球菌感染对不同品系罗非鱼血液生化指标和肝脏HSP70 mRNA表达的影响[J].水产学报,2012,36(6):958-968,11.基金项目
公益性行业(农业)科研专项(200903046-02) （农业）
现代农业产业技术体系建设专项资金“罗非鱼产业技术体系”(CARS-49) （CARS-49）
江苏省普通高校研究生科研创新计划(CXLX11-0708) （CXLX11-0708）
中央公益性科研业务费专项资金(2011jbfa09) （2011jbfa09）
广东省海洋渔业科技推广专项[A201009C02,A2010002-010(b)] （b）
广东省科技计划项目(2010B090500032) （2010B090500032）
