山东农业大学学报（自然科学版）2012，Vol.43Issue(2)：163-168,178,7.
过量表达甜菜BvNHX1基因提高拟南芥的耐盐性
OVEREXPRESSION OF BVNHX1 FROM BETA VULGARIS TO IMPROVE SALT TOLERANCE IN ARABIDOPSIS THALIANA
王鹏 1安静 2侯蕾 1孔祥强 2赵彦修 2张慧1
作者信息
- 1. 山东省农业科学院,山东济南 250100
- 2. 山东师范大学、山东省逆境植物重点实验室,山东济南 259014
- 折叠
摘要
Abstract
Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. In order to reveal the function of BvNHXl from Beta vulgaris in plant salt resistance, the plant expression vector pROK H - BvNHXl was constructed and introduced into Arabidopsis thaliana, Transformants were selected for their ability to grow on a medium containing kanamycin. Southern blot analyses confirmed lhat BvNHXl was transferred into the Arabidopsk thaliana genome and northern blot analyses showed that BvNHXl was expressed in the transformants. Several transgenic homozygous lines and wild - type plants were evaluated for salt tolerance, the results suggest that transgenic Arabidopsis thaliana plants expressing BvNHXl improved seed germination rate and salt tolerance in seedlings. Under saline conditions, both fresh and dry weights of the trans-genics as well as their accumulations of Na + and + were much higher than that of wild - type. These results demonstrate that overexpression of BvNHXl in Arabidopsis thaliana increased salt tolerance of transgenic plants compared with the wild - type plants.关键词
盐生植物/BvNHX1/液泡膜Na+/H+逆向转运蛋白/耐盐性/拟南芥Key words
Halophyte/ BvNHXl/ vacuolarNa+/H+ antiporter/ salt tolerance/ Arabidopsis thaliana分类
农业科技引用本文复制引用
王鹏,安静,侯蕾,孔祥强,赵彦修,张慧..过量表达甜菜BvNHX1基因提高拟南芥的耐盐性[J].山东农业大学学报（自然科学版）,2012,43(2):163-168,178,7.
