山东农业大学学报（自然科学版）2012，Vol.43Issue(2)：206-210,219,6.
山东地区樱桃绿环斑驳病毒(CGRMV)的RT-PCR 检测及外壳蛋白基因的克隆
DETECTION OF CHERRY GREEN RING MOTTLE VIRUS (CGRMV) AND ISOLATION OF ITS COAT PROTEIN GENES FROM SWEET CHERRY IN SHANDONG PROVINCE
摘要
Abstract
In order to investigate the infection of Cherry Green Ring Mottle Virus (CGRMV) from sweet cherry ( Prunus avium L. ) in Shandong province, total RNA was extracted from the leaves of the sweet cherry cultivar Red Lamp' The specific primers were designed corresponding to the CGRMV genome sequence and used for the RT - PCR detection. Thirty - seven sweet cherry samples from six orchards were analyzed in the experiment and nineteen of them were detected as positive. The gene that encodes the virus coat protein was amplified and se-quenced. Sequence analysis revealed that the fragment was 807 nucleotides in length, encoding 268 amino acids. It shared 87% ~97% identity to the other CGRMV isolates reported in GenBank in the nucleotide level and 95% ~99% similarity in the amino acids level. These results showed CGRMV had been wide spread on sweet cherry in Shandong district.关键词
甜樱桃/樱桃绿环斑驳病毒/RT -PCR检测/外壳蛋白/基因克隆Key words
Prunus avium L. / Cherry green ring mottle virus/ RT - PCR detection/ coat protein/ gene cloning分类
农业科技引用本文复制引用
王文文,宗晓娟,陈立伟,王甲威,魏海蓉,徐丽,孟艳玲,严雪瑞,刘庆忠..山东地区樱桃绿环斑驳病毒(CGRMV)的RT-PCR 检测及外壳蛋白基因的克隆[J].山东农业大学学报（自然科学版）,2012,43(2):206-210,219,6.基金项目
农业部948项目国外甜樱桃抗逆种质资源的引进创新及产业化(2011-Z40) （2011-Z40）
公益性行业(农业)科研专项樱桃产业主要障碍因素攻关研究(200903019) （农业）
山东省农业良种工程甜樱桃新品种和抗性砧木选育[鲁农良种字(2010)6号] （2010）
