山东农业科学2012，Vol.44Issue(5)：1-4,4.
陕西核桃实生居群遗传多样性ISSR分析
ISSR Analysis of Genetic Diversity of Shanxi Walnut (Juglans regia) Populations
摘要
Abstract
The genetic diversity of 39 individuals and genetic structure of the population of Shanxi walnut ( Juglans regia L. ) were analyzed by inter - simple sequence repeat markers (ISSR). Eight primers were selected from 13 pre -screened primers for PCR. With them, 48 polymorphic loci were detected with the percentage of polymorphic bands ( PPB) as 87.27%. The ISSR - derived genetic similarity (ISSR - GS) ranged from 0.473 ~0. 836 with a mean of 0. 679. The Shannon - Weaver index of genetic diversity ranged from 1.483 to 1.960 and the Simpson index ranged from 0.739 to 0.846. The UPGMA cluster analysis showed certain similarity to it, but also presented obvious differences. In conclusion, the ISSR markers could separate the 39 individuals, which provided theoretical reference and technical support for the scientific protection and utilization of walnut resources.关键词
实生核桃/资源/居群遗传结构/ISSRKey words
Natural walnut/ Resources/ Population genetic structure/ ISSR分类
生物科学引用本文复制引用
陈新,张士刚,魏海蓉,宗晓娟,王甲威,刘庆忠..陕西核桃实生居群遗传多样性ISSR分析[J].山东农业科学,2012,44(5):1-4,4.基金项目
科技部科技基础工作资助项目"核桃、板栗资源圃标准化整理、整合及共享试点"(2006DKA21002-20) （2006DKA21002-20）
