山东医药2012，Vol.52Issue(15)：12-14,3.
波动性高糖对INS-1细胞凋亡及凋亡相关基因表达的影响
Effects of intermittent high glucose on the apoptosis of INS-1 cell and expression of apoptosis-related gene
摘要
Abstract
Objective To investigate the effects of intermittent high glucose on the apoptosis of INS-1 cell and apopto-sis-related gene expression, and discuss its potential molecular mechanism. Methods The INS-1 beta cells were cultured and randomly divided into three groups. Nonnal control group(NG group) : containing 5.5 nunol/L glucose; sustained high glucose group(SHG group) : containing 33. 3 mmol/L glucose; intermittent high glucose group( IHG group) : containing 5.5 mmol/L or 33.3 mmol/L glucose, exchanged the culture medium every 24 hours. All the three groups were cultured for three days. Cell viability, percentage of apotosis, insulin secretion, protein expressions of Caspase-3, CytC and Bax and the mRNA levels of gene related to glucose metabolism were detected. Results Compared with the NG group, the apoptosis rate of SHG group and IHG group increased significantly, and the cell viability decreased obviously, the expressions of Bax, CytC and Caspase-3 increased, the insulin secretion well as the mRNA expressions of insulin and PDX-I gene reduced(all P <0.01). Compared with the SHG group, the cell viability and insulin secretion of IHG group decreased significantly , the apoptosis and the protein expression of Caspase-3 increased obviuusly{ all P < 0.01). There was no statistically significant difference on the expression of CytC and Bax as well as the mRNA levels of insulin and PDX-1 gene. Conclusions Intermittent high glucose can lead to increased apoptosis and dysfunction of islet beta cells, (he mechanism may be associated with increase of the expression of Bax, CytC, caspase-3 and decrease of the expressions of insulin and PDX-1 gene.关键词
波动性高糖/胰岛素瘤细胞/细胞凋亡/基因表达Key words
intermittent high glucose / apoptosis/ gene expression分类
医药卫生引用本文复制引用
邓珊珊,刘红,荣曦,岳惠芬,张君,吴宇娟..波动性高糖对INS-1细胞凋亡及凋亡相关基因表达的影响[J].山东医药,2012,52(15):12-14,3.基金项目
国家自然科学基金资助项目(31160189) （31160189）
广西壮族自治区教育厅科研项目(201012MS052) （201012MS052）
广西壮族自治区研究生教育创新课题项目(2011105981002M228) （2011105981002M228）
广西壮族自冶区科技厅回国基金课题(桂科回0991006 （）
2009CM4005). （）
