BDNF基因修饰的NSCs移植对大鼠脊髓损伤后轴突再生及后肢运动功能的影响OACSTPCD
Effects of neural stem cell modified by BDNF transplantation on the axonal regeneration and function of hind limbs in rats after spinal cord injury
目的 探讨脑源性神经营养因子(BDNF)基因修饰的神经干细胞(NSC-BDNF)移植对脊髓损伤(SCI)的可行性及效果.方法 将120只SCI大鼠随机分为四组各30只,假手术组(Sham组)仅做椎板切开术,伤后3d损伤组(SCI组)脊髓内注射生理盐水溶液5μl,NSC组髓内注射移植细胞密度为2×105/μl的NSCs悬液5μl,NSC-BDNF组髓内注射NSC-BDNF悬液5μl.采用免疫组织化法检测1、3、7、14、28 d脊髓组织BDNF、神…查看全部>>
Objective To investigate the effects of neural stem cell (NSC)modified by brain derived neurophic factor (BDNF) transplantation on the axonal regeneration and function of hind limbs in rats after spinal cord injury( SCI). Methods Total of 120 SCI rats were divided randomly into 4 groups with 30 in each,the Sham groupreceived laminotomy only, the 3 d after SCI group was given 5 μL normal saline by intraspinal injection, the NSC group was given 5 μL cell suspe…查看全部>>
张国庆;樊立华;徐红梅;孙奎兴
青岛大学医学院附属医院,山东青岛266100东营市人民医院青岛大学医学院附属医院,山东青岛266100青岛大学医学院附属医院海阳分院
医药卫生
脊髓损伤脑源性神经营养因子神经干细胞轴突再生后肢功能
spinal cord injury brain derived neurophic factor neural stem cell axonal regeneration hind limb function
《山东医药》 2012 (15)
48-50,3
青岛市科技发展计划资助项目(10-3-1-7-1-jch).
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