山东医药2012，Vol.52Issue(17)：4-6,9,4.
Kisspeptin-GPR54-GnRH神经元轴在雌性大鼠中枢性性早熟中的作用
Effect of Kisspeptin-GPR54-GnRH neuron axis in central precocious puberty of female rats
摘要
Abstract
Objective To study the effect of Kisspeptin-GPR54-GnRH neuron axis in central precocious puberty (CPP) of female rats. Methods Fifty female rats were randomly assigned to five groups, the first control group (normal prepuberty) , the second control group (early phase of normal puberty) , the third control group (metaphase of normal puberty) , the first experimental group (early phase of precocious puberty), the second experimental group (metaphase of precocious puberty) , ten rats in each group. Experimental group received subcutaneous injection of N-methyl-DL-aspartale acid (NMA) in order to establish the central precocious puberty model. The vaginal open and estrous cycle of all rats were observed. The parameters of puberty were analyzed, which including index of ovary, index of uterus, incidence of corpora lutea, thickness of uterus and level of serum luleinizing hormone. The expressions of hypothalamic KISS-1 mRNA, GPR54 mRNA and GnRH mRNA were detected by Real-Time reverse transcription polymerase chain reaction (Real-Time RT-PCR). Ultrastructure of hypothalamic endocrinic neurons was observed by electron microscope. Results The day of puberty onset in experimental groups was earlier than that of the control groups. The parameters in experimental groups were higher than those of the first control group. The parameters in the first experimental group were not obviously different from the second control group, as well as the second experimental group versus the third control group. With puberty process, the expressions of hypothalamic KISS-1 mRNA, GPR54 mRNA and GnRH mRNA in experimental or control groups all gradually increased. The hypothalamic endocrinic neurons were active in metabolism with prosperous secretion. Conclusion The central precocious puberty model of female rats could be perfectly established by way of NMA subcutaneous injection. It is suggested that Kisspeptin-GPR54-GnRH neuron axis may play an important part in onset and development of CPP in view of the increase of KISS-1 mRNA, GPR54 mRNA and GnRH mRNA expressions in hypothalamus with puberty process.关键词
性早熟,中枢性/青春期/KISS-1/GPR54/GnRH/大鼠Key words
precocious puberty, central/ puberty/ KISS-1/ G protein-couple receptor 54/ gonadotropin releasing hormone/ rats分类
医药卫生引用本文复制引用
王海莲,葛伟,薛江..Kisspeptin-GPR54-GnRH神经元轴在雌性大鼠中枢性性早熟中的作用[J].山东医药,2012,52(17):4-6,9,4.基金项目
山东省自然科学基金资助项目(Y2007C137). （Y2007C137）
