山东医药2012，Vol.52Issue(17)：32-34,3.
不同用药顺序吉非替尼、多西他赛联用对非小细胞肺癌细胞的体外抑制作用
Sequence-dependent antiproliferative effects of gefitinib combined with docetaxel in non-small cell lung cancer cells in vitro
摘要
Abstract
Objective To explore the sequence-dependent antiproliferative effects of gefltinib combined with docetaxel in non-small cell lung cancer cells in vitro. Methods PC-9, PC-9/C and A549 cell lines were chosed. The cell survival rates were measured by MTT assay, cell cycle distribution and apoptosis were measured by flow cytometry docetaxel. According to the sequence of administration of gefitinib combined with docetaxel, the cells were divided into docetaxe followed by gefitinib group ( D-G group) and gefitinib followed by docetaxel group ( G-D group). Results Compared with the G -D group, D-G group remarkably decreased cell survival, induced Ga-M phase arrest and enhanced apoptosis induced by docetaxel; in contrast, G-D group induced G0-G1 phase arrest and decreased apoptosis induced by docetaxel. Conclusion Sequential administration of docetaxel followed by gefitinib is optimal combination schedule for the antiproliferative effects of non-small cell lung cancer cells.关键词
癌,非小细胞肺/吉非替尼/多西他赛/细胞存活率/细胞周期/细胞凋亡Key words
carcinoma, non-small-cell lung/ gefitinib/ docetaxel/ cell survival/ cell cycle/ apoptosis分类
医药卫生引用本文复制引用
玄香兰,周彩存,安昌善..不同用药顺序吉非替尼、多西他赛联用对非小细胞肺癌细胞的体外抑制作用[J].山东医药,2012,52(17):32-34,3.基金项目
国家自然科学基金资助项目(81160291). （81160291）
