山东医药2012，Vol.52Issue(18)：4-6,3.
浙贝母碱对肺癌A549/DDP细胞多药耐药的逆转作用观察及机制探讨
Efficiency and mechanisms of peimine reversing multi-drug resistance of A549/DDP cell line
摘要
Abstract
Objective To explore the effect and mechanism of reversing multi-drug resistance( MDR) in adenocarci-noma line A549/DDP by peimine. Methods The AS49/DDP cells were divided into two groups. The cells in control were given different concentration of cisplatin, and the cells in expremental group were given 400 μg/mL peimine on the control group basis. MTT assay was used to detect the cell proliferation ratio,and IC50 and reversing multipe were calculated. The A549/DDP cells were divided into blank control group(nothing) .control group(cisplatin) and experimental group (cispla-lin + 400 μg/mL peimine). After 48 h, apoptosis was anlysed by Dow cytometry, expression of resistance associated protein LRP was detected by cell immunofluorescence technique. Results IC50 of cisplatin in experimental group was 4. 15μg/ mL,and 15. 46 μg/mL in control group,the reversing multipe was 3.73. After 48 hours, cell apoptosis rate among blank contron) group ,contronl group and experimental group was respectively 2.56 ± 0.44,13.5 ± 1.42,38.16 ± 2.25, and the expression of LRP of each group was (9.8 ± 1.92)/HP, (9.2 ± 1.9)/HP, (5.8 ±1.3)/HP. There were much more cell apoptosis and less expression were in the experiment group than in the control group (P < 0.05). Conclusions Peimine could reverse MDR of A549/DDP cell line. The mechanism may be reletived with inducing apoptosis and down-regulating LRP expression.关键词
浙贝母碱/细胞增殖/细胞凋亡/人肺耐药蛋白/多药耐药Key words
peimine/ proliferation/ apoptosis/ receptor related protein/ multi-drug resistance分类
医药卫生引用本文复制引用
唐晓勇,唐迎雪..浙贝母碱对肺癌A549/DDP细胞多药耐药的逆转作用观察及机制探讨[J].山东医药,2012,52(18):4-6,3.基金项目
山东省中医管理局科研计划资助项目(2009-170). （2009-170）
