实验动物与比较医学2012，Vol.32Issue(2)：142-145,4.DOI:10.3969/j.issn.1674-5817.2012.02.013
一种以小鼠精子为主导的综合性保种体系
A Comprehensive Method of Strain ConservationMediated by Spermatozoa in Mice
摘要
Abstract
Objective To establish a comprehensive system for mouse strain conservation through natural mating, sperm cryopreservation and assisted reproductive technology. Methods Mouse fresh epididymal sperm were collected by vasectomy of unilateral testis and the vasectomized mice were naturally mated for breeding. If no satisfactory results obtained from natural breeding, then the cryopreserved sperm were thawed for In Vitro Fertilization (IVF). If no results obtained, Intracytoplas-mic Sperm Injection (ICSI) was used to reconstructed embryos and which were finally transferred into pseudopregnant recipient female mouse. Results The results show that parts of mice vasectomized unilateral testis such as BDF1 and C57BL/6J mice could be used for natural breeding. Among the remaining sterile males, only ICR mice could be preserved through IVF while ICSI technology was the last approach to save GFP mice with C57BL/6J background. Conclusion The genetic background of mouse sperm cryopreservation was a key factor in IVF and setup a comprehensive system, which could achieve the purpose of strain conservation while mouse strains could be utilized at maximum extent.关键词
小鼠/单侧睾丸摘除/体外受精/胞质内单精子注射/品系保存Key words
Mouse/ Vasectomy of Unilateral Testis/ In Vitro Fertilization/ Intracytoplasmic Sperm Injection/ Strain conservation分类
生物科学引用本文复制引用
李敦高,蒋满喜,陈学进,李和平..一种以小鼠精子为主导的综合性保种体系[J].实验动物与比较医学,2012,32(2):142-145,4.基金项目
上海市科学技术委员会科研计划项目(编号:09140901000和10140901700) （编号:09140901000和10140901700）
