同济大学学报（医学版）2012，Vol.33Issue(2)：38-42,5.DOI:10.3969/j.issn1008-0392.2012.02.008
活性维生素D3抑制大鼠间质成纤维细胞激活作用的研究
1,25-dihydroxyvitamin D3 suppresses fibronectin production induced by TGF-β1 in renal interstitial fibroblasts
李瑾 1李微 1殷少军 1陈维杰1
作者信息
- 1. 同济大学附属第十人民医院老年科,上海200072
- 折叠
摘要
Abstract
Objective To investigate the effect of 1,25-(OH)2VitD3 on the production of fibronectin induced by transforming growth factor β1 (TGF-β1) in renal interstitial fibroblasts. Methods To study the effect of 1,25-(OH)2VitD3 and TGF-β1 on ECM synthesis. The renal interstitial fibroblast NRK-49F cells were divided into control group, TGF-β1 group, VitD3group and TGF-β1 plus VitD3 group. The de novo expression of α-smooth muscle actin (α-SMA) and fibronectin were detected by western blot analysis and immunofluorescence. Results TGF-β1 induced de novo expression of α-SMA and production of fibronection in a time-dependent manner. TGF-β1 also affected assembly of α-SMA and deposition of fibronectin. 1,25-( OH)2VitD3 alone had no effects on a-SMA and fibronectin expression.Whereas, 1,25-( OH)2VitD3, portially obliterated T expression of α-SMA and fibronectin in a dose-dependent manner. Conclusion The results indicate that l,25-(OH)2VitD3 can partially inhibit renal interstitial fibroblast activation and fibronectin synthesis induced by TGF-β1.关键词
活性维生素D3/成纤维细胞/转化生长因子β1/纤维连接蛋白Key words
1,25-(OH)2VitD3/ fibroblast/ transforming growth factor β1/ fibronectin分类
医药卫生引用本文复制引用
李瑾,李微,殷少军,陈维杰..活性维生素D3抑制大鼠间质成纤维细胞激活作用的研究[J].同济大学学报（医学版）,2012,33(2):38-42,5.
