同济大学学报（医学版）2012，Vol.33Issue(2)：43-46,4.DOI:10.3969/j.issn1008-0392.2012.02.009
miR-145在肝癌中的表达和功能研究
miR-145 expression in hepatic carcinoma and its effects on cell proliferation and invasion of tumor
胡臻 1李佳 2房林2
作者信息
- 1. 同济大学附属第十人民医院麻醉科,上海200072
- 2. 同济大学附属第十人民医院甲状腺乳腺外科,上海200072
- 折叠
摘要
Abstract
Objective To investigate the expression of miR-145 in hepatic carcinoma, adjacent normal hepatic tissues and HepG2 cell line, and its effect on proliferation and invasion of human hepatic carcinoma. Methods RT-PCR was performed to determine the expression of miR-145 in hepatic carcinoma, adjacent normal liver tissues and human hepatocellular carcinoma HepG2 cells. Proliferation, invasion assays were conducted to examine the effects of miR-145 on HepG2 cells through up-regulating miR-\45 expression by miR-145 mimics. Results Compared to normal hepatic tissues, miR-145 expression was decreased in liver cancer specimens and HepG2 cells (P <0. 05). Proliferation assay revealed that 100 nmol/L miR-145 significantly inhibited HepG2 cell growth at 72 h. A significantly different number of invaded HepG2 cells were observed between groups treated by 100 nmol/1 miR-\45 and controls. Conclusion The expression of miR-145 is decreased in hepatic carcinoma and HepG2 cell line. Up-regulation of miR-145 expression inhibits cell growth and invasion of HepG2 cells in vitro.关键词
肝肿瘤/微小RNA-145/增殖/侵袭Key words
hepatic carcinoma/miR-145/ proliferation/ invasion分类
医药卫生引用本文复制引用
胡臻,李佳,房林..miR-145在肝癌中的表达和功能研究[J].同济大学学报（医学版）,2012,33(2):43-46,4.
