生命科学研究2012，Vol.16Issue(2)：113-119,7.
水稻逆境响应基因OsMsr15的表达分析与克隆
Expression and Cloning of A Stress Responsive Gene OsMsr15 in Rice
摘要
Abstract
By using the GeneChip Rice Genome Array (Affymetrix), global genome expression profiles of rice Pei'ai 64S were analyzed at seeding and booting stages under heat, drought and cold stresses. A number of stress responsive genes were identified. OsMsr\5 was highly reduced in response to heat, drought and cold stresses, which was matched to the results from microarray and quantitative real-lime RT-PCR analyses. Sequence analysis showed that the ORF of OsMsr\5 is 717 bp in length, encods a protein of 238 amino acids with M. W≈2.46 kD and pI≈8.90. The gene had no intron, and the encoded protein contained typical conserved C2H2 domain. A putative nuclear location signal (B-box) and an ERF-associated amphiphilic repression motif (EAR-motif) were found in the N-terminal and C-terminal regions of the protein, respectively. Analysis of the putative promoter region found that several regulatory elements related to stress responses . Proteins with high similarity to 0sMsr15 were found among different species. Thus, OsMsr\5 might be a conserved candidate gene involved in plant stress tolerance.关键词
水稻/逆境/基因芯片/基因克隆Key words
Oryza saliva L./ stress/ microarray/ gene cloning分类
生物科学引用本文复制引用
张新,陈荣军,王曼玲,宋书锋,夏新界..水稻逆境响应基因OsMsr15的表达分析与克隆[J].生命科学研究,2012,16(2):113-119,7.基金项目
中国科学院"百人计划"项目(02200420062903) （02200420062903）
