生命科学研究2012，Vol.16Issue(2)：120-124,5.
水稻基结合蛋白基因MBD701的克隆及原核表达
Cloning of Methyl-binding Domain Protein Gene MBD701 and Its Prokaryotic Expression
摘要
Abstract
MBD is a kind of trans-acting factor which binding with DNA methylation, which plays important regulation roles during the growth and development in plant. In order to study the structure and function, the MBD701 in rice was cloned by using the RT-PCR, the prokaryotic expression vector pGEX-MBD701 was constructed and transformed to E.coli. BL21 (DE3). The amino acid sequence analysis showed that MBD701 contained a conserved methyl-CpG binding domain, and CW-zinc finger as well. The fusion protein GST-MBD701 with molecular weight of 65.87 kD was effectively expressed under 1 mmol/L IPTG (Isopropyl-beta-D- thiogalactopyranoside) concentrations at 37℃, which provides a foundation for further purification and functional study of the MBD701 protein.关键词
水稻/甲基结合蛋白/基因克隆/原核表达Key words
rice/ methyl-binding domain protein/ gene cloning/ prokaryotic expression分类
生物科学引用本文复制引用
张艳霞,贾海英,司志飞,刘昊英,孟凡荣..水稻基结合蛋白基因MBD701的克隆及原核表达[J].生命科学研究,2012,16(2):120-124,5.基金项目
国家自然科学基金资助项目(30300195) （30300195）
