生命科学研究2012，Vol.16Issue(2)：138-143,148,7.
番茄多蛋白桥梁因子基因LeMBF1的克隆及抗病性分析
Cloning and Analysis of Pathogen Resistance of Multiprotein Bridging Factor Gene LeMBF1 in Tomato
摘要
Abstract
The full-length cDNA of LeMBFl was cloned from tomato by Homology-based cloning method, which contained an ORF of 420 bp and encoded a protein of 139 amino acid residues containing a conserved MBFl transcription activation domain. Compared with Solatium tuberosum StMBF1, Nkotiana tabacum NtMBF1 and Vitis vinifera VvMBFl , the deduced amino acid sequence of LeMBFl shares 99.3%, 91.4% and 84.2% similarity, respectively. To analyze the pathogen resistance of multiprotein bridging factor gene LeMBFl in tomato, the LeMBF1-overexpressing tomatoes and wild type lines were inoculated with Pst. DC3000 and Yusarium. oxysporum respectively. The phenotypic analysis of pathogen resistance displayed that the plaque number on the LeNBFl-overexpressing tomato leaves was significantly less than the control plants. qRT-PCR analysis showed that the expression levels of pathogenesis-related PRl and PR6 genes were significantly increased in LeMBFl -overexpessing tomato plants. These results indicated that LeMBFl elevated the pathogen resistance of tomato plants by activating the expression of some PR genes.关键词
番茄/多蛋白桥梁因子/LeMBF1/抗病性Key words
tomato/ multiprotein bridging factor/ LeMBFl/ pathogen resistance分类
农业科技引用本文复制引用
唐平华,陈国平,潘宇,张彦杰,张彬,胡宗利..番茄多蛋白桥梁因子基因LeMBF1的克隆及抗病性分析[J].生命科学研究,2012,16(2):138-143,148,7.基金项目
国家自然科学基金资助项目(31000911) （31000911）
中央高校基本科研业务项目(CDJXS10230035) （CDJXS10230035）
