生命科学研究2012，Vol.16Issue(2)：164-168,5.
miRNA沉默ILK基因对人膀胱癌T24细胞侵袭功能的影响
The Effect of Bladder Cancer Invasion Function Through miRNA Silence Integrin Linked Kinase(ILK) Gene
摘要
Abstract
To explore the effect of (he invasion function of bladder cancer cell T24 after gene silenced integrin linked kinase(ILK) gene. ILK miRNA(micro RNA) were constructed, then transfected into bladder cancer cell lines T24. The change of ILK gene and protein was delected by RT-PCR (reverse transcriptase-poly-raerase chain reaction) and Western blot. The change of invasion function were measured by heterogeneous adhesion experiment and Transwell. The ILK gene and protein was decreased after ILK miRNA was transfected. The A value of heterogeneity adhesion was 0.21 ±0.05 in miRNA plasmid group. The A value of heterogeneity adhesion was 0.67+0.08 and 0.63±0.04 in negative plasmid group and untransfected group (P＜ 0.05). The cell number of invasion was (7.2±1.1) cells/HP in miRNA plasmid group, but in negative plasmid group and untransfected group was (15.4±1.6) cells/HP and (13.2±1.2) cells/HP (P＜0.05). The results indicated that silencing ILK would decrease the invasion fuction of bladder cancer cell line T24.关键词
整合素连接激酶/膀胱癌/转移/侵袭Key words
integrin linked kinase/ bladder cancer/ metastasis/ invasion分类
医药卫生引用本文复制引用
易正金,何云锋,王勇,高飞,雷波..miRNA沉默ILK基因对人膀胱癌T24细胞侵袭功能的影响[J].生命科学研究,2012,16(2):164-168,5.基金项目
重庆市卫生局基金资助项目(2011-2-103) （2011-2-103）
教育部博士点新教师基金资助项目(20115503120012) （20115503120012）
