生命科学研究2012，Vol.16Issue(3)：242-247,6.
胰蛋白酶消化法和组织块法原代培养人包皮成纤维细胞的比较
Comparing Trypsin Digestion and Tissue Piece Method in Primary Culture of Human Foreskin Fibroblasts
王志强 1梁锐 2陈明清 1邓俊 3李文亮 1陈天星 2文政琦 1杨军1
作者信息
- 1. 昆明医科大学第一附属医院肿瘤科,中国云南昆明650032
- 2. 云南省第一人民医院病理科,中国云南昆明650032
- 3. 昆明医科大学第一附属医院男性科,中国云南昆明650032
- 折叠
摘要
Abstract
At present, it is recognized internationally that mouse embryonic fibroblasts (MEFs) can be used as the feeder-layer of human embryonic stem cells (hESCs) in vitro culture. But MEFs have defects such as short life, animal-derived contamination. Therefore, human-derived feeder-layer which owns long life and is suitable for hESCs in vitro culture needs to be explored. Trypsin digestion and tissue piece method were adopted respectively to carry on primary culture of human foreskin fibroblasts (hFFs). The best primary culture method of hFFs was explored and it will provide evidence for the application of hFFs in hESCs study. Morphology and characteristic of hFFs obtained by both primary culture methods were verified with inverted microscope and immunocytochemical stain. Results of cell growth curve and MTT method displayed high proliferative ability in hFFs obtained by both primary culture methods at different passages. Good morphology of hFFs can be maintained after 10 passages. Thus hFFs obtained by these two primary culture methods can be applied in the study of hESCs.
关键词
人包皮成纤维细胞/原代培养/胰蛋白酶消化法/组织块法/饲养层/人胚胎干细胞Key words
human foreskin fibroblasts/ primary culture/ trypsin digestion method/ tissue piece method/feeder-layer/ human embryonic stem cells分类
医药卫生引用本文复制引用
王志强,梁锐,陈明清,邓俊,李文亮,陈天星,文政琦,杨军..胰蛋白酶消化法和组织块法原代培养人包皮成纤维细胞的比较[J].生命科学研究,2012,16(3):242-247,6.
