柱前衍生高效液相色谱-荧光检测法测定血浆中同型半胱氨酸OA北大核心CSCDCSTPCD
Determination of homocysteine in plasma by precolumn derivatization-high performance liquid chromatography with fluorescence detection
建立了一种柱前衍生高效液相色谱-荧光检测法用于测定血浆中同型半胱氨酸(Hcy).使用三(2-羧乙基)膦盐酸盐(TCEP)为还原剂,N-(1-芘)马来酰亚胺(NPM)为衍生剂进行样品预处理,Agilent Hypersil C-18柱(250mm ×4.0 mm,5μm)进行分离,流动相为15 mmol/L醋酸钠-乙腈-混合酸(300 mL水中含1mL醋酸和1mL磷酸)混合溶液,采用梯度洗脱,荧光检测激发波长为330nm,发射波长为380 nm.H…查看全部>>
A precolumn derivatization-high performance liquid chromatographic method for the determination of homocysteine (Hey) in plasma was established. Tris (2-carboxyethyl) phos-phine hydrochloride (TCEP) and N-( 1-pyrenyl) maleimide (NPM) were used as the reduced reagent and derivatization reagent, respectively. The separation was carried out on an Agilent Hypersil C-18 column (250 mm x4. 0 mm, 5μm) in gradient elution mode. The mobile phase consisted of A (15 mm…查看全部>>
唐秀芳;甄乾娜;樊子勉;冯成亚;丁敏
重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016重庆医科大学附属第一医院内分泌内科,重庆400016重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016重庆医科大学检验医学院,临床检验诊断学教育部重点实验室,重庆400016
化学化工
高效液相色谱荧光检测同型半胱氨酸血浆
high performance liquid chromatography ( HPLC ) fluorescence detectionhomocysteine plasma
《色谱》 2012 (6)
613-617,5
重庆市科委自然科学基金项目(No.CSTC2011JJA0225).
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