色谱2012，Vol.30Issue(6)：630-634,5.DOI:10.3724/SP.J.1123.2012.02015
反相高效液相色谱法同时测定化妆品中7种萘二酚类物质
Simultaneous determination of seven naphthalenediols in cosmetics by reversed-phase high performance liquid chromatography
摘要
Abstract
A novel analytical method for the simultaneous determination of seven naphthalenediols ( 2,6-naphthalenediol, 1,5-naphthalenediol, 1,6-naphthalenediol, 2,7-naphthalenediol, 1,7-naphthalenediol, 1,3-naphthalenediol and 2,3-naphthalenediol) in cosmetics by reversed-phase high performance liquid chromatography (RP-HPLC) with a diode array detector was established. The moisturizer, cream, emulsion and water-like cosmetic samples were extracted with 95% ethanol (v/v), and the1 powder-like cosmetic samples were extracted with 95% etha-nol-0. 1% acetic acid (3=2, v/v), then the extracts were centrifuged, filtrated, and analyzed by RP-HPLC using a C18 column, employing methanol-0. 1% acetic acid aqueous solution as the mobile phases under the condition of gradient elution. The naphthalenediols were qualitatively determined by retention times, and confirmed by ultraviolet spectra. The external standard method was applied for the quantification. The results indicated that the limits of quantification (LOQs) of the seven naphthalenediols were ranged from 0. 5 mg/kg to 1. 2 mg/kg (S/N = 10); meanwhile, the linear correlation coefficients of them were all no less than 0. 999 0 within the linear ranges. Their recoveries in spiked samples at the levels of 5. 0 - 50 mg/kg were ranged from 84. 0% to 102% with the relative standard deviations (RSDs) of 1. 3% - 5. 7% (n = 6). The method is simple, precise and suitable for the determination of naphthalenediols in cosmetic samples.关键词
反相高效液相色谱法/萘二酚/化妆品Key words
reversed-phase high performance liquid chromatography ( RP-HPLC)/ naphthalenediols/ cosmetics分类
化学化工引用本文复制引用
陈立坚,黄金凤,何敏恒,林森煜,郭新东..反相高效液相色谱法同时测定化妆品中7种萘二酚类物质[J].色谱,2012,30(6):630-634,5.基金项目
广东省地方标准计划项目(No.[2010]299). （No.[2010]299）
