色谱2012，Vol.30Issue(6)：635-640,6.DOI:10.3724/SP.J.1123.2011.12086
改进的离子色谱法测定乳制品中亚硝酸盐和硝酸盐
Determination of nitrite and nitrate in dairy products by improved ion chromatography
摘要
Abstract
Nitrite and nitrate are common inorganic salts in the diet and drinking water. It is generally believed that excessive intake of these substances may result in methemoglobinemia or other diseases. However, the traditional detection methods for nitrite and nitrate in dairy products restrain their applications to routine analysis due to the presence of certain limitations. In order to solve this problem, an improved national food safety standard method for the determination of nitrite and nitrate in dairy products has been studied. After water extraction, protein precipitation and centrifugation, the supernatant was cleaned up by a solid phase extraction (SPE) column. The eluent mainly composed of sodium hydroxide with acetonitrile as organic modifier. External water mode was used for suppressor. An AS 19 column was used as the analytical column, and the oven temperature was 30℃ while the cell temperature was 35 Xi. The detection wavelength was 225 nm and the injection volume was 200μL. The results showed that good linearity existed when the concentrations of nitrite and nitrate were between 0. 005 -0. 50 and 0. 05 - 1. 50 mg/L respectively. The detection limits of nitrite and nitrate were 0. 2 and 0. 04 mg/kg respectively when using a conductivity detector; while the values were only 0.02 and 0.01 mg/kg using an ultraviolet (UV) detector. The recoveries were between 84. 0% and 104. 1% when analyzing dairy products. It is a simple, fast and highly sensitive way for nitrite and nitrate detections.关键词
离子色谱/电导检测/紫外检测/亚硝酸盐/硝酸盐/乳制品/改进/国家标准方法Key words
ion chromatography/ conductivity detection/ ultraviolet detection/ nitrite/ nitrate/dairy products/ improve/ national standard method分类
化学化工引用本文复制引用
钟莺莺,陈平,俞雪钧,许迪明,房科腾,彭锦峰..改进的离子色谱法测定乳制品中亚硝酸盐和硝酸盐[J].色谱,2012,30(6):635-640,6.基金项目
宁波市自然科学基金项目(No.2011A610007). （No.2011A610007）
