中国生态农业学报2012，Vol.20Issue(5)：592-596,5.DOI:10.3724/SP.J.1011.2012.00592
东北黑土区大豆根际促生菌群落组成研究
Community structure of soybean plant growth-promoting rhizobacteria in the black soil region of Northeast China
摘要
Abstract
As the black soil area of Northeast China is major soybean producing areas, it is important for the sustainable development of agriculture to study the soybean plant growth-promoting rhizobacteria (PGPR). For explaining the community structure of soybean PGPR in the black soil region of Northeast China, we chose four sample stations including Ewenki Autonomous Banner in Inner Mongolia Autonomous Region, Hailun City in Heilongjiang Province, Keshan County in Heilongjiang Province and Hongxinglong Farms in Heilongjiang Province, analyzed community composition of nitrogen-fixing bacteria, phosphate-dissolving bacteria, phosphate-solubilizing bacteria and silicate bacteria in soybean rhizosphere, and interpreted correspondence relationship between PGPR strains and areas. The results showed a large number of PGPR in soybean rhizosphere. The quantity of nitrogen-fixing bacteria reached to 104 cfu·g-1, these of phosphate-dissolving bacteria and phosphate-solubilizing bacteria reached to 105 cfu·g-1, and that of silicate bacteria reached to 103 cfu·g-1. Species groups of nitrogen-fixing bacteria, phosphate-dissolving bacteria, phosphate-solubilizing bacteria and silicate bacteria in soybean rhizosphere consisted of 5, 6, 7 and 4 species respectively. Shannon-Weiner biodiversity indices of nitrogen-fixing bacteria, phosphate-dissolving bacteria, phosphate-solubilizing bacteria and silicate bacteria were higher in soybean rhizosphere, which were in ranges of 0.94～1.60, 0.83～1.52, 1.07～1.67 and 0.52～0.96.Biodiversity indices of PGPR were more than 2 in sampling stations. Analysis on correspondence relationship between PGPR strains and areas indicated typical PGPR of different sampling stations. Nitrogen-fixing bacteria LLN8 (Azotobacter beijerinckia indica), phosphate-dissolving bacteria DHS13 (Micrococcus), phosphate-dissolving bacteria DHS19 (Pseudomonas), nitrogen-fixing bacteria LLN1 (A. Chrooco-ccum) and phosphate-dissolving bacteria DHS5 (Azotobacter) were typical PGPR strains for Ewenki Autonomous Banner in Inner Mongolia Autonomous Region, Hailun City in Heilongjiang Province, Keshan County in Heilongjiang Province, Hongxinglong Farms in Heilongjiang Province, respectively. The paper cleared that some strains including LLN2 (A. Azomonas), LLN6 (B. Mucilaginosus), DHS9 (Arthrobacter), DHSO2 (Pseudomonas), DHSO14 (Erwinia), DHSO17 (Corynebacterium) and LSJ21 (Bacillus) were widespread in the black soil area of Northeast China through correspondence analysis, the strains could serve as an important foundation of special compound bio-fertilizer production.关键词
东北黑土区/大豆/根际促生菌/群落结构/多样性Key words
Black soil region of Northeast China, Soybean, Plant growth-promoting rhizobacteria (PGPR), Community structure, Diversity分类
农业科学引用本文复制引用
王志刚,徐伟慧,莫继先,肖静,孙剑秋,王建丽..东北黑土区大豆根际促生菌群落组成研究[J].中国生态农业学报,2012,20(5):592-596,5.基金项目
黑龙江省教育厅科学技术研究项目(11551539)和齐齐哈尔大学青年科研启动支持计划(2010k-M10)资助 （11551539）
