山西医科大学学报2012，Vol.43Issue(8)：581-583,638,4.DOI:10.3969/J.ISSN.1007-6611.2012.08.007
荧光金逆行标记乳鼠视网膜神经节细胞的实验研究
Research on retinal ganglion cells of neonatal rats labeled retrogradely with fluorogold
摘要
Abstract
Objective To establish a method to label the retinal ganglion cells of neonatal rats with fluorogold. Methods One-day neonatal Sprague-Dawley rats were anesthetized by intraperitoneal injection with chloral hydrate, and then 1 μ1 of 4% fluorogold was mi-croinjected bilaterally into the superior colliculi of rats. Four days later, primary mixed retinal cell cultures were established from retinas of five-day neonatal rats. Results Under fluorescence microscope, retinal ganglion cells in mixed cultured retinal cells were successfully labeled with fluorogold, which accounted for 0. 34% of total retinal cells. In addition, retinal ganglion cells survived for three weeks in vitro. Conclusion Retinal ganglion cells of neonatal rats labeled by fluorogold is a feasible approach, and could contribute to the I-dentification of retinal ganglion cells in primary mixed retinal cell cultures.关键词
荧光金/乳鼠/视网膜神经节细胞Key words
fluorogold/ neonatal rats/ retinal ganglion cells分类
生物科学引用本文复制引用
李静波,徐尤学,方家华,张祖海..荧光金逆行标记乳鼠视网膜神经节细胞的实验研究[J].山西医科大学学报,2012,43(8):581-583,638,4.基金项目
国家自然科学基金资助项目(81000380) （81000380）
湖北省卫生厅青年科技人才基金资助项目(QJX2010-53) （QJX2010-53）
湖北省荆州市科技局基金资助项目(2010-2)再注射另一侧,双侧均注射完 （2010-2）
