天然产物研究与开发2012，Vol.24Issue(6)：729-735,7.
多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究
Antifungal Lipopeptide Produced by Paenibacillus polymyxa HY96-2
摘要
Abstract
Isolation and purification of antifungal substance from Paenibacillus polymyxa HY96-2,a commercial biocon-trol strain used in control of fusarium wilt disease, was studied in this paper. By means of acidification,n-BuOH extraction, ethyl acetate precipitation, silica gel column chromatography, sephadex LH-20 column chromatography and HPLC preparation ,6B was isolated and purified. Its structure was elucidated as fusaricidin A by means of spectroscopic analysis including NMR,MS and ESI-MS/MS. Results of two-fold serial agar dilution method showed that the minimum inhibition concentrations of 6B against 15 phytopathogenic fungi were 12.5 -50 μg/mL. Greenhouse results showed that 6B had better control effect on Botrytis cinerea of cucumber. The control effect could reach ;5% when its concentration was set at 250 μg/mL.关键词
多粘类芽孢杆菌/抗真菌活性/分离纯化/结构鉴定/Fusaricidin AKey words
Paenibacillus polymyxa/ antifungal activity /solation and purification/ structure identification/ fusaricidin A分类
化学化工引用本文复制引用
范磊,张道敬,刘振华,陶黎明,罗远婵,魏鸿刚,李淑兰,李元广..多粘类芽孢杆菌HY96-2产脂肽类抗真菌物质的研究[J].天然产物研究与开发,2012,24(6):729-735,7.基金项目
国家农业科技成果转化资金项目(2007GB2C000101) （2007GB2C000101）
国家863项目(2006AA10A211) （2006AA10A211）
上海市重点学科建设项目(B505) （B505）
