天然产物研究与开发2012，Vol.24Issue(6)：736-740,767,6.
蒺藜皂苷对糖基化终产物形成及其诱导的内皮细胞功能障碍影响研究
Effects of Saponins from Tribulus terrestris L.on Nonenzymatic Glycation and Endothelial Cell Dysfunction
摘要
Abstract
Previous researches have confirmed that saponins from Tnbulus terrestris L. (STT) is effective in controlling serum glucose and lipid levels. The present study investigated the effect of STT on advanced glycation end products (ACEs) formation and AGEs-mediated endothelial cell dysfunction. Fluorescence detection was used to monitor the Maillard reaction. Primary cultured bovine aortic endothelial cells (BAECs) were exposed to 100 (μg/mL AGEs for 30 min followed by STT supplementation. Cell viability was detected by MTT assay,whereas cellular nitric oxide (NO) release, enzymatic activity of NO synthase (NOS) ,and levels of superoxide were monitored by reagent kit. Data showed that STT increased the formation of ACEs at 10 μLg/mL. The supplementation of STT also accelerated the decrease in BAECs viability regardless of cell exposure to AGEs. Furthermore, STT elevated the AGEs-induced decline in NO release, as well as increased inducible NOS (iNOS) activities and superoxide levels. Compared with the effects of hecoge-nin and tigogenin,the negative effect of STT on AGEs-injured BAECs might be ascribed to hecogenin. Our findings suggested that STT was not a suitable candidate for inhibiting nonenzymatic glycation,and it accelerated the AGEs-mediated endothelial cell dysfunction probably by enhancing iNOS activity.关键词
糖基化终产物/内皮细胞/皂苷/一氧化氮/一氧化氮合酶/超氧阴离子Key words
advanced glycation end products/endothelial cells/saponins/nitric oxide/nitric oxide synthase/superoxide分类
农业科技引用本文复制引用
庄秀园,陈原国,曹晶晶,瞿伟菁,张雯..蒺藜皂苷对糖基化终产物形成及其诱导的内皮细胞功能障碍影响研究[J].天然产物研究与开发,2012,24(6):736-740,767,6.基金项目
国家自然科学基金(30370158) （30370158）
