天然产物研究与开发2012，Vol.24Issue(6)：741-746,753,7.
复合生物保鲜剂对松鼠葡萄球菌的抑菌性能及其作用机理研究
Antimicrobial Activity and Mechanism of Complex Biological Fresh-keeping Agents against Staphylococcus sciuri
摘要
Abstract
In this paper,gram-positive Staphyhcoccus sciuri was selected to study the antimicrobial activity and mechanism of complex biological fresh-keeping agents (CBFA) ,which was composed of chitosan 10.0 g/L,lysozyme 0.3 g/L and tea polyphenol 3.0 g/L. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of CBFA against S. sciuri were determined by oxford plate assay system, and the effects of CBFA with different concentration on bacteria in different time were studied by cell damage experiments,such as antimicrobial kinetics,alkaline phos-phatase ( AKP) activity,the integrity and permeability of cell membrane, and ultra microstrueture of bacteria. The results showed that the MIC and MBC of CBFA against S. sciuri were 0.8 and 1.6 mg/mL. With the extension of treated time, the growth of bacteria was significantly inhibited, the level of AKP increased, the membrane became permeable, the integrity of the cellular structure was undermined, and the electronic conductivity and ultraviolet absorbance of extracellular fluid increased. S. sciuri began to shrink,distorted,surface rough,the cell walla were sunk,the cytoplasm leaked from cells and led to cell death ultimately.关键词
复合生物保鲜剂/壳聚糖/溶菌酶/茶多酚/松鼠葡萄球菌/抑菌机理Key words
complex biological fresh-keeping agents (CBFA) /chitosan/lysozyme/tea polyphenol/Staphylococcus sciuri/antimicrobial mechanism分类
生物科学引用本文复制引用
蓝蔚青,谢晶,侯伟峰,李达文..复合生物保鲜剂对松鼠葡萄球菌的抑菌性能及其作用机理研究[J].天然产物研究与开发,2012,24(6):741-746,753,7.基金项目
“十二五”国家支撑计划项目(2011BAD24B02) （2011BAD24B02）
上海海洋大学博士启动基金(A-2400-11-0001-0202) （A-2400-11-0001-0202）
上海市教育委员会重点学科建设项目(J50704) （J50704）
教育部高等学校博士学科点专项科研基金(20113104110005) （20113104110005）
