天然产物研究与开发2012，Vol.24Issue(6)：800-803,859,5.
高速逆流色谱分离制备川西獐牙菜中两种苷类化合物
Preparative Separation of Two glycosides from Swertia Mussotti by High-Speed Counter-Current Chromatography
摘要
Abstract
A high performance method for isolation and purification of two glycosides from Swertia mussotti was successfully established by utilizing high-speed counter-current chromatography ( HSCCC). Separation was performed with a two-phase solvent system composed of n-butanol-chloroform-methanol-water (3.4:8:5 :6,v/v). 1-O-primevenosyl-3,7, 8-trimethoxyxanthone (11 mg) and isoorientin (24 mg) were isolated and purified in one-step elution from 100 mg crude sample under the conditions of a flow rate 1.5 mL/min in the initial 210 min followed by 2.5 mL/min,800 r/min and the detection wavelength of 254 run. HPLC analysis showed that the purity of two compounds was over 99%. Their chemical structures were confirmed by UV,1H- and 13C NMR.关键词
川西獐牙菜/高速逆流色谱/1-O-樱草糖-3,7,8-三甲氧基(口山)酮/异荭草苷Key words
Swertia mussotti/HSCCC/1-O-primever-osyl-3,7,8-trimethoxyxanthone/isoorientin分类
生物科学引用本文复制引用
陈涛,贾静,王萍,尤进茂,刘永军,李玉林..高速逆流色谱分离制备川西獐牙菜中两种苷类化合物[J].天然产物研究与开发,2012,24(6):800-803,859,5.基金项目
国家自然科学基金项目(20875099) （20875099）
中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26) （KSCX2-EW-J-26）
