西北林学院学报2012，Vol.27Issue(4)：117-123,7.DOI:10.3969/j.issn.1001-7461.2012.04.22
葡萄SVP类MADS-box基因的克隆及表达分析
Cloning and Expression Analysis of Grape SVP-like MADS-box Gene
摘要
Abstract
A MADS-box gene associated with ovule development was cloned from Vitis. vinifera cv. Thompson seedless. The full-length of cDNA gene was 1 248 bp, including a 729 bp open reading frame, and encoding 243 amino acids. The homology and phylogenetic analysis showed that this gene belonged to SVP-like MADS-box genes. The gene was expressed in all of the grape tissue type examined by semi-quantity RT-PCR, including root, stem, leaf, bud. full bloom and ovules, but varied widely in the level of expression. Real-time PCR analysis revealed that gene expression level during ovule development in Thompson increased firstly and then decreased. In contrast to this, gene expression level in Pinot showed downward trend in all stages of development.关键词
葡萄/SVP类MADS-Box基因/表达分析Key words
Vitis/ SVP-like MADS-box gene/ expression analysis分类
农业科技引用本文复制引用
杨堃,张朝红,李树秀,张旭彤,王跃进..葡萄SVP类MADS-box基因的克隆及表达分析[J].西北林学院学报,2012,27(4):117-123,7.基金项目
国家自然科学基金项目(31171926) （31171926）
国家现代农业产业技术体系建设专项基金(CARS-30-yz-7) （CARS-30-yz-7）
公益性行业(农业)科研专项(200903044) （农业）
