畜牧兽医杂志2012，Vol.31Issue(4)：8-9,13,3.
间接免疫荧光方法快速检测番鸭呼肠孤病毒病
Rapid Detecting Muscovy Duck Reovirus by Indirect Fluorescence Assay
摘要
Abstract
Monoclonal antibody（MDRV 237--11）against Muscovy duck reovirus was used to build up indirect fluorescence assay（IFA） in order to detect Muscovy duck reovirus （MDRV）. The results showed that frozen section of Muscovy duck infec- ted with MDRV MW9710 strain could appear specific bright green fluorescence by IFA, moreover, 12--old--day Muscovy duck embryo inoculated with liver and spleen homogenate were isolated the virus. So, IFA could replace virus isolation to detect Muscovy duck reovirus rapidly.关键词
番鸭呼肠孤病毒病/间接免疫荧光试验/快速检测Key words
Muscovy duck reovirus/indirect fluorescence assay/rapid detecting分类
农业科技引用本文复制引用
高春亮,蔡羲,朱小丽,陈少莺..间接免疫荧光方法快速检测番鸭呼肠孤病毒病[J].畜牧兽医杂志,2012,31(4):8-9,13,3.基金项目
福建省农业科学院创新团队（STIF-Y02）、福建省畜牧重大专项（2006NZ003）、省公益类项目（201IRl025-7）和福建省星火计划 （）
