烟草科技Issue(5)：44-47,4.
采用全烟气暴露系统测试卷烟主流烟气细胞毒性
In vitro Cytotoxicity Assay for Mainstream Cigarette Smoke with Whole Smoke Exposure System
摘要
Abstract
The method of in vitro cytotoxicity assay for mainstream cigarette smoke was established by using VITROCELL? whole smoke exposure system associated with neutral red uptake assay. The cytotoxicity of mainstream cigarette smoke of 29 domestic and imported commercial available brands was evaluated by this method under Health Canada Intensive (HCI) smoking regime. The results indicated that: 1) A well dose-response relationship between cigarette smoke doses and cell viability could be obtained by the established method. 2)The stability of the method was satisfactory with the values of CV below 25%. The established method of whole smoke exposure could be applied to evaluate the cytotoxicity of mainstream cigarette smoke.关键词
卷烟烟气/全烟气/HCI抽吸条件/中性红/细胞毒性Key words
Cigarette smoke/ Whole smoke/ HCI smoking regime/ Neutral red/ Cytotoxicity分类
轻工纺织引用本文复制引用
李翔,聂聪,尚平平,谢复炜,刘惠民,谢剑平..采用全烟气暴露系统测试卷烟主流烟气细胞毒性[J].烟草科技,2012,(5):44-47,4.基金项目
国家自然科学基金资助项目“卷烟主流烟气和侧流烟气中颗粒度的尺度、化学组成与生物毒性研究”(21007094),国家烟草专卖局科技项目“卷烟危害性指标体系深化研究”(110200801022),国家烟草专卖局标准项目“卷烟全烟气暴露的体外毒理学测试方法”(2011B056). （21007094）
