云南农业大学学报2012，Vol.27Issue(3)：315-320,6.DOI:10.3969/j.issn.1004-390X(n).2012.03.003
水稻细菌性条斑病菌的实时荧光PCR检测技术研究
Real-time Quantitative PCR for Detection of Xanthomonas oryzae pv.oryzicola
摘要
Abstract
Xanthomonas oryzae pv.oryzicola was listed as a plant quarantine disease of China.Primers pair of Xoc2071 F/Xoc2071 R ( Xoc2071) is specific primers based on cysteine synthase gene to amplify fragment 329 bp.Using SYBR Green I fluorescence quantitative PCR method and primers Xoc2071,the detection method of Xooc by qPCR was established.The results showed thai the primers Xoc2071 could specifically detect the target bacteria,but the other pathogenic bacteria can not have fluorescence signals.The level of detection was 2.25 fg/μL plasmid DNA and 1.0 ×102 cfu/mL bacterial suspen-sion,roughly equaling to one cell and 100 times sensitive than PCR gel electrophoresis detection.Xooc was detected from seed washes of infected seeds as small as 10 g.关键词
水稻细菌性条斑病菌/检测/荧光定量PCRKey words
Xanthomonas oryzae pv. oryzkola/detection/fluorescence quantitative PCR (qPCR)分类
植物保护学引用本文复制引用
韩阳,张丽辉,王永吉,林琳,周丽洪,姬广海..水稻细菌性条斑病菌的实时荧光PCR检测技术研究[J].云南农业大学学报,2012,27(3):315-320,6.基金项目
农业部公益性(农业)行业科研专项(3-23) （农业）
公益性行业(农业)科研专项(201003029). （农业）
