云南农业大学学报2012,Vol.27Issue(3):346-352,7.DOI:10.3969/j.issn.1004-390X(n).2012.03.008
日粮不同能量和蛋白质水平对乌金猪肌肉组织能量代谢的影响
Effect of Dietary Energy and Protein Levels on Energy Metabolism of Muscle Tissue in Wujin Pigs
摘要
Abstract
The aim of the study was to investigate the effects of dietary energy and protein levels on energy metabolism of muscle tissue in Wujin pigs.One hundred and eight Wujin pigs were randomly allotted into six groups fed diet containing different energy or protein levels.There were eighteen pigs in each group with three replicates which contained six pigs for each replicate.The dietary energy and protein levels were high energy (HE,14.22 MJ/kg),medium energy ( ME,12.89 MJ/kg) and low energy (LE,11.74 MJ/kg),high protein (HP,18%,16% and 14%),medium protein ( MP,16%,14% and 12% ),low protein (LP,14%,12% and 10% ) respectively.At the body weight of 30,60 and 100 kg,Wujin pigs were slaughtered and blood,liver and muscular tissues were taken for analyzing contents of blood sugar,hepatic glycogen,muscle glycogen,lactic acid,and activity of lac-tate dehydrogenase,Na+ K +-ATPase and Ca2+ Mg2 +-ATPase.The results showed that contents of blood sugar,hepatic glycogen,muscle glycogen and lactic acid and the activity of Na+ K+-ATPase,Ca2+ Mg2 +-ATPase and lactate dehydrogenase increased as Wujin pigs fed dietary of energy or protein level increased.The higher dietary energy or protein level could increase the contents of blood sugar,hepatic glycogen,muscle glycogen and lactic acid and the activity of Na+ K+-ATPase,Ca +Mg +-ATPas,and lactate dehydrogenase at 60kg body weight (P <0.05).At the body weight of 100 kg,the higher dietary energy or protein level could increase contents of blood sugar,hepatic glycogen and muscle glycogen and the activity of Na+ K+-ATPase and Ca2 +Mg2 -ATPase.The results indicated that higher energy ( HE,14.22 MJ/kg) or protein (HP,18%) level of dietary could promote synthesis of hepatic glycogen and muscle glycogen,increase the action of glycolytic pathway,and therefore advanced the energy metabolism of muscle tissue in Wujin pigs.关键词
乌金猪/日粮能量水平/日粮蛋白水平/肌肉组织/能量代谢Key words
Wujin pigs/dietary energy level/dietary protein level/muscle tissue/energy metabolism分类
畜牧业引用本文复制引用
潘洪彬,李长强,黄英,张永云,赵素梅,葛长荣,张曦,高士争..日粮不同能量和蛋白质水平对乌金猪肌肉组织能量代谢的影响[J].云南农业大学学报,2012,27(3):346-352,7.基金项目
云南省自然科学基金重点项目(2005C0008Z) (2005C0008Z)
云南省自然科学基金项目(2011FB052). (2011FB052)